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KMID : 0613820050150010124
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Journal of Life Science
2005 Volume.15 No. 1 p.124 ~ p.131
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Studies on Intracellular Functions of the mas3+ Gene in the Fission Yeast, Schizosaccharomyces pombe
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Hwang Mi-Ra
Cha Jae-young
Shin Sang-Min
Park Jong-Kun
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Abstract
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The regulation of gene expression plays an important rolet in cell cycle controls. In this study, a novel mas3+ (mitosis associated protein) gene, a homolog of human SMARCAD1, was isolated and characterized from a fission yeast Schizosaccharomyces pombe. The overall homology between the helicase proteins of the two species is 87%. This DEAD/H box-containing molecule has seven highly conserved sequence regions that allow us to place it in the SNF2 family of the helicase superfamily. Knock-out cell of mas3+ gene was constructed using kanMX6 as a selection marker. Survival of mas3 null mutant exposed to UV or MMS was similar to those of wild type cells. mas3+ expression was lowest at G₂and gradually increased. Cytokinesis of mas3 null mutant was abnormal at 26℃ and 35℃ and a large number of multi-septate cells were produced. These results indicate that the mas3+ is involved in cytokinesis and cell shape control.
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KEYWORD
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